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. 2017 Nov 10;18(11):2386. doi: 10.3390/ijms18112386

Figure 6.

Figure 6

The binding sites and abilities of NRF2 and BACH1 to the HO-1 promoter. (A) Diagram of putative ARE enhancer sites in the HO-1 promoter regions for NRF2 and BACH1 binding and primers used for the ChIP assays; (B) ChIP assay analysis for binding of NRF2 and BACH1 to the HO-1 gene ARE. HBE1 cells after smoke treatment were collected at 6 h, fixed in formaldehyde, and ChIP analysis was performed using IgG, NRF2 and BACH1 antibodies. The immunoprecipitated chromatin was PCR-amplified, run on agarose gel and photographed. The mean enrichment intensities are shown in the bottom panel as fold relative to that of untreated HBE1 cells (n = 3; mean ± S.D.).