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. 2017 Nov 11;18(11):2396. doi: 10.3390/ijms18112396

Figure 1.

Figure 1

Identification of differentially expressed microRNAs and potential microRNA–mRNA interactions in rheumatoid arthritis primary osteoblasts. (A) The next generation sequencing (NGS) identified 35 differentially expressed microRNAs (thresholds of >2.0-fold change and reads per million (RPM) >10) in rheumatoid arthritis (RA) osteoblasts, compared to normal osteoblasts. The heat map analysis with z-score values is shown here. (B) The 16 up-regulated and 19 down-regulated microRNAs predicted 435 and 391 putative targets, respectively, using the miRmap database with selection threshold of miRmap score ≥99.0. Additionally, 434 protein-coding genes with >2.0-fold change and >0.3 fragments per kilobase of transcript per million (FPKM) were identified from the NGS, where 199 genes were up-regulated and 235 genes were down-regulated in RA osteoblasts. The putative targets of up-regulated (down-regulated) microRNAs were matched to the down-regulated (up-regulated) protein-coding genes by the Venn diagram analysis. Finally, thirteen genes (eight down-regulated and five up-regulated) with potential miRNA–mRNA interactions were identified.