Figure 6.

Expression of (A) thymic stromal lymphopoietin (TSLP) protein and (B) TSLP receptor (TSLPR) protein in normal and burn-scar tissues with or without pruritus was measured by (C) western blotting (WB). Expression of (D) TSLP mRNA was measured using quantitative real-time PCR in normal and burn-scar tissues with or without pruritus. The greatest TSLP expression of mRNA and protein was observed in pruritic burn-scar tissue using qPCR and western blotting (A–C). TSLPR proteins were also highest in pruritic burn-scar tissue via western blotting results (D). NC: keratinocytes from normal control; B (N): keratinocytes from nonpruritic burn scar; B (P): keratinocytes from pruritic burn scar; TSLP: thymic stromal lymphopoietin; TSLPR: thymic stromal lymphopoietin receptor; Error bars: standard deviation of the mean value obtained from three experiments, each performed in triplicate. * p < 0.001.