Table 1.
List of group II intron and their splicing efficiencies in nmat2, pmh2 and double nmat2/pmh2 mutants.
| Mitochondrial Group II Introns | Introns Configuration | nMAT2-Dependent | PMH2-Dependent | Splicing Affected in nmat2/pmh2 |
|---|---|---|---|---|
| ccmFc i1 | cis | No a,c | No b, ambiguous c | No |
| cox2 i1 | cis | Yes a,c | Yes b,c | Yes |
| nad1 i1 | trans | No a,c | No b,c | No |
| nad1 i2 | cis | Yes a,c | Yes b,c | Yes |
| nad1 i3 | trans | ambiguous a, Yes c | Yes b,c | Yes |
| nad1 i4 | cis | No a,c | No b,c | ambiguous |
| nad2 i1 | cis | ambiguous a, Yes c | Yes b,c | Yes |
| nad2 i2 | trans | No a,c | Yes b,c | Yes |
| nad2 i3 | cis | No a,c | No b,c | No |
| nad2 i4 | cis | ambiguous a, Yes c | Yes b,c | Yes |
| nad4 i1 | cis | No a,c | No b,c | No |
| nad4 i2 | cis | ambiguous a, Yes c | Yes b,c | Yes |
| nad4 i3 | cis | No a,c | Yes b,c | Yes |
| nad5 i1 | cis | ambiguous a, Yes c | Yes b,c | Yes |
| nad5 i2 | trans | ND a, Yes c | Yes b,c | Yes |
| nad5 i3 | trans | ND a, Yes c | Yes b,c | Yes |
| nad5 i4 | cis | ambiguous a, No c | No b, ambiguous c | No |
| nad7 i1 | cis | No a,c | Yes b,c | Yes |
| nad7 i2 | cis | Yes a,c | No b, ambiguous c | Yes |
| nad7 i3 | cis | ambiguous a, No c | No b,c | ambiguous |
| nad7 i4 | cis | ambiguous a, No c | Yes b,c | Yes |
| rpl2 i1 | cis | ambiguous a,c | Yes b,c | Yes |
| rps3 i1 | cis | ambiguous a, Yes c | Yes b,c | Yes |
a, splicing defects in nmat2 mutants as indicated in Keren et al. [33]; b, reduced splicing efficiencies in pmh2 mutants as indicated by Köhler et al. [37]; c, splicing defects supported by the transcriptome data and RT-qPCR analyses (this study); Grey shaded columns indicate to decreased splicing efficiencies in the double nmat2/pmh2 mutants; ND, Not determined.