Figure 4.

Pathogen infection of GhMAP3K65-overexpressing plants. (a,e) Symptoms in detached leaves of WT and GhMAP3K65-overexpressing plants in response to R. solanacearum and R. solani infection, respectively. The detached leaves were photographed 7 days after infection; (b,f) Symptoms in detached leaves of WT and GhMAP3K65-overexpressing plants following R. solanacearum and R. solani infection, respectively. The accumulation of H₂O₂ was detected via 3,3′-diaminobenzidine (DAB) staining; (c) Bacterial growth in the leaves of WT and GhMAP3K65-overexpressing plants after R. solanacearum infection; (g) The number of spores per cotyledon after R. solani infection; (d,h) Chlorophyll contents were measured after R. solanacearum and R. solani infection, respectively. The experiments were repeated at least three times. Different letters above the columns indicate significant differences (p < 0.05) according to Duncan’s multiple range test.