Figure 4.

Yeast two-hybrid spot test results for all four proteins and positive and negative controls on quadruple drop-out medium (QDO)/X-α-gal + 3AT (5 mM) plates. For the spot test, each strain bearing the plasmid was grown in 3 ml of appropriate drop out liquid media at 30 °C and shaken at 280 rpm for 2 days. To reconfirm the interaction, 10 ΜL of each culture (washed and resuspended in 0.9% w/v NaCl) was mixed and spotted on QDO plates containing X-α-gal at the indicated dilutions and incubated at 30 °C for 3–5 days. Undil, undiluted; 10× and 100× dilutions.