Figure 7.

Effect of quercetin on the phosphorylation of MAPKs and Akt in VEGF-stimulated 661W cells. Cells were treated with VEGF (20 ng/mL) in the absence or presence of quercetin (0.1 µM) for 1 h. The phosphorylation levels of ERK (A), JNK (B), p38 (C), and Akt (D) were detected by immunoblot using corresponding antibodies. Densitometry quantifications of protein expression were measured by ImageJ. Data are the means ± SDs of three independent experiments. * p < 0.05 and ** p < 0.01 indicate significant differences compared to the non-treated control group. # p < 0.05 and ## p < 0.01 indicate significant differences compared to the VEGF-only treated group.