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. 2017 Dec 4;10:288. doi: 10.1186/s13068-017-0976-9

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — LC–MS profiles of the reaction catalyzed by recombinant Hno from A. tumefaciens S33. The HPLC profile was obtained by monitoring with a PDA detector at 210 nm; a–c the mass spectra of the substrate 6-hydroxynicotine (m/z 179.1) and the products 6-hydroxy-N-methylmyosmine (m/z 177.1) and 6-hydroxypseudooxynicotine (m/z 195.1), respectively. Positively charged ions were detected