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. 2017 Dec 4;10:288. doi: 10.1186/s13068-017-0976-9

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Biotransformation of nicotine into 6-hydroxynicotine catalyzed by the whole cells of A. tumefaciens S33-∆hno. a HPLC profile of the reaction mixtures sampled at different time after starting the reaction. The increase and decrease of the peaks during the reaction are indicated by upward or downward arrows, respectively. b Time course of the biotransformation reaction