Skip to main content
. 2017 Nov 10;199(12):4091–4102. doi: 10.4049/jimmunol.1701030

FIGURE 3.

FIGURE 3.

Engagement of TIM-3 by Ab increases transcription of genes involved in T cell effector function with concomitant decrease in genes associated with T cell memory. Ag-specific CD8 T cells were restimulated as described in Fig. 1. Total RNA was extracted from T cells treated with either anti–TIM-3 mAb or isotype control and analyzed by RNA sequencing. (A) Data were evaluated using IPA and the top upstream regulators are shown with predicted activation state, z-score, p value, and gene transcript levels of downstream targets that are increased (green) or decreased (red). (B) A p value cutoff of 0.05 and fold change ≥2 (up- and downregulation) was used to classify transcripts as differentially expressed in treatment condition. Data representative of two donors.