FIGURE 6.

Combined engagement of TIM-3 and PD-1 does not impact T cell function. Ag-specific CD8 T cells were cultured as in Fig. 1 in the presence of anti–TIM-3 mAb, anti–PD-1 mAb, or anti–TIM-3 and anti–PD-1 combined, and analyzed for (A) proliferation by flow cytometry and (B) cytokine production. (C) Representative dot plots showing expression of CCR7 versus CD45RA expression and MART1 tetramer expression on Ag-specific CD8 T cells (*p < 0.0001, two-way ANOVA.) Data shown are representative of four to six biological replicates per treatment condition performed in three independent experiments.