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. 2017 Nov 28;4:139–151. doi: 10.2147/JHC.S139070

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© 2017 Suryatenggara et al. This work is published and licensed by Dove Medical Press Limited

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Flow cytometry histograms showing the expression of functional surface molecules of DC.

Notes: Groups of DC (imDC, imDC-AFP, mDC, and mDC-AFP) from one of the biological samples were treated with fluorescence-conjugated antibody against five functional surface molecules of DC (HLA-DR, CD40, CD83, CD80, and CD86) and read by flow cytometry. Percentage of cells with positive expression was calculated relative to the total number of DC read by flow cytometer. (A) Flow cytometry histogram of HLA-DR. (B) CD40. (C) CD83. (D) CD80. (E) CD86. Gating was determined based on the fluorescence values of cells in mDC group, in which the respective surface molecules are at their expected normal expression level after LPS maturation. Cells with a value below the threshold were considered to suboptimally express each respective surface molecule and was denoted in each dot plot as negative cells. Positive cell percentage shown in each dot plot was calculated relative to the total number of DC read by the flow cytometer.

Abbreviations: DC, dendritic cell; imDC, immature DC; imDC-AFP, alpha-fetoprotein-treated imDC; mDC, mature DC; mDC-AFP, alpha-fetoprotein-treated mDC; LPS, lipopolysaccharide.