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. 2017 Nov 28;10:5667–5675. doi: 10.2147/OTT.S143084

Figure 4.

Figure 4

MxA is a direct target of miR-211.

Notes: (A) TargetScan revealed that the 3′-UTR of MxA mRNA contains a putative site that is partially complementary to miR-211. (B) Luciferase activity assay revealed that miR-211 suppressed wild-type MxA 3′-UTR luciferase activity, while it had no effect on mutant MxA 3′-UTR luciferase activity compared to control in A549 cells. (C) Ectopic miR-211 expression inhibited MxA protein expression in A549 cells. (D) qRT-PCR analysis was used to measure the expression of MxA in 40 NSCLC tissues and 40 adjacent noncancerous tissues. (E) Western blot analysis was used to measure the expression of MxA in 40 NSCLC tissues and 40 adjacent noncancerous tissues. (F) qRT-PCR analysis was used to measure the expression of MxA in four NSCLC cell lines (A549, H1650, SPC-A1, and NCI-H460) and one human bronchial epithelial cell line (16HBE). (G) The mRNA expression of MxA correlated inversely with miR-211. ***P<0.001; **P<0.01; *P<0.05.

Abbreviations: miR-211, microRNA-211; miRNA, microRNA; NSCLC, non-small-cell lung cancer; qRT-PCR, quantitative real-time polymerase chain reaction; UTR, untranslated region.