Fig. 1. The overall structure of the PaTrm5a-tRNAPhe-SAH ternary complex and the multiple sequence alignment of the representatives from other species.
(A) Biosynthetic pathways for wyosine derivatives in archaea including P. abyssi. Note that the same enzyme Trm5 performs two methyltransfers and is marked in red. (B) The front and back views of the complex in ribbon rendition. The D1, D2, and D3 domains are colored pale green, yellow orange, and light blue, respectively. The active site SAM is shown as spheres, whereas tRNA is in orange. The linker connecting D1 and D2 is colored blue.