Skip to main content
. 2017 Nov 30;8:2041731417741505. doi: 10.1177/2041731417741505

Figure 5.

Figure 5.

Confinement of primary hepatocytes cultured on stiff- and soft-PDMS/APTES/GA scaffolds patterned with COL I in lines. (a) Freshly isolated rat hepatocytes were seeded at high density (2 × 105 cells per well) on stiff- and soft-PDMS/APTES/GA scaffolds patterned by microcontact printing with type I collagen micropatterns. Primary rat hepatocytes viability was evaluated by calcein AM/PI assay. Live cells are shown in green and dead cells are shown in red. Differential interference contrast (DIC) and confocal microscopy images were acquired 24 h after cell culture. Scale bars = 100 μm. (b) Albumin (cytosol) and HNF4-α (nucleus) specific markers were expressed in primary rat hepatocytes cultured for 24 h at high density (2 × 105 cells per well) on stiff- and soft-PDMS/APTES/GA scaffolds patterned with 1 mg/mL COL I in lines. Scale bars = 20 μm. (c) Primary rat hepatocytes were cultured for 24 h at high density (2 × 105 cells per well) on stiff- and soft-PDMS/APTES/GA scaffolds coated with 1 mg/mL type I collagen. Confocal microscopy images show subcellular localisation of albumin (cytoplasm) and HNF4-α (nucleus) specific biomarkers of primary cultured hepatocytes. Nuclei were stained with DAPI. Scale bars = 20 μm.