Figure 2.

miR-124-3p protects SH-SY5Y cells against MPP⁺-induced neuronal injury. (a) MTT assay determined the cell viability in SH-SY5Y cells treated with different concentrations of MPP⁺ (0, 0.25, 0.5, 1, 2 mM). SH-SY5Y cells were transfected with miR-124-3p mimics prior to 1 mM MPP⁺ treatment for 24 h. (b) miR-124-3p improved the viability of SH-SY5Y cells treated with MPP⁺. (c) The apoptosis of MPP⁺-treated SH-SY5Y cells was inhibited by miR-124-3p. (d) miR-124-3p reduced the Caspase-3 activity in SH-SY5Y cells treated with MPP⁺. (e) miR-124-3p decreased the LDH activity in SH-SY5Y cells treated with MPP⁺. (f and g) The level of TNF-α and IL-1β in the supernatant of MPP⁺-treated SH-SY5Y cells was lowered by miR-124-3p. (h) miR-124-3p prevented ROS generation in SH-SY5Y cells treated with MPP⁺. (i) miR-124-3p promoted the SOD activity in SH-SY5Y cells treated with MPP⁺. Significant differences were assessed using one-way ANOVA. Multiple comparisons between the groups were performed using S-N-K method. *P < 0.05.

MPP: methyl phenyl pyridinium iodide.