Table 1.
Details of antibodies and concentrations used.
| Antibody | Supplier | Product code | Batch | Epitope | Concentration used |
||
|---|---|---|---|---|---|---|---|
| WB | ICC | Co-IP | |||||
| mGlu3 | Abcam | ab166608 | YJ100911CS | 845-C terminus | 1:100,000 | 1:5000 | – |
| mGlu3 | Abcam | ab188750 | GR230043-1, GR230043-2 | N-terminal domain | 1:1000 | ||
| V5 HRP conjugated | Invitrogen | 46-0708 | 861217 | V5 | 1:10,000 | – | – |
| V5 FITC | Invitrogen | 46-0308 | 1819586 | V5 | 1:1000 | ||
| mGlu2/3 | Millipore | AB1553 | 1652168 | C-terminus with glutaraldehyde (NGREVVDSTTSSL) | – | – | 1:50 |
| N-cadherin | BD Biosciences | 610920 | 78545 | – | 1:10,000 | – | – |
| GAPDH | Abcam | ab9484 | GR165366-3 | – | 1:5000 | – | – |
| α-tubulin | Abcam | ab7291 | GR200985-3 | aa 426–450 | – | 1:2000 | – |
| HRP goat anti-rabbit | Biorad | 172-1019 | 350003011 | – | 1:10,000 | – | – |
| HRP goat anti-mouse | Biorad | 172-1011 | 350003068 | – | 1:5000 | – | – |
| Alexa 568 goat anti- rabbit | Invitrogen | A11011 | 623962 | – | – | 1:1000 | – |
| Alexa 488 goat anti-mouse | Invitrogen | A11001 | 632115 | – | – | 1:1000 | – |
| Alexa 488 donkey anti-mouse | Invitrogen | A10037 | 1696197 | – | – | 1:1000 | – |
Co-IP: co-immunoprecipitation; FITC: fluorescein isothiocyanate; HRP: horseradish peroxidase; ICC: immunocytochemistry; WB: western blot; aa: amino acids.