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. 2017 Sep 25;45(20):11962–11979. doi: 10.1093/nar/gkx845

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — eIF2Bα, eIF2Bβ, and eIF2B_reg bind eIF2α-NTD and eIF2α-NTD_S51D with similar affinities. Plot of the changes in fluorescence anisotropy of fluorescein-labeled eIF2α-NTD (black) and eIF2α-NTD_S51D (purple) in the presence of increasing concentrations of unlabeled eIF2Bα (solid squares) and eIF2B_reg (circles) (A), and eIF2Bβ (B). Calculated K_Ds are shown next to the corresponding titration curves. Zoomed-in eIF2B_reg titration curves are shown in the inset of panel A. No substantive difference in affinity was observed for eIF2Bα, eIF2Bβ, or eIF2B_reg as a function of the phosphorylation state of eIF2α-NTD.