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. 2017 Sep 28;45(20):11782–11799. doi: 10.1093/nar/gkx871

Figure 5.

Figure 5.

3E10 inhibits RAD51 nuclear localization and foci formation after irradiation. (A) Primary skin fibroblasts were left untreated or pretreated with purified 2XMBP-scFv proteins and then irradiated with 10Gy IR and fixed for immunofluorescence. (B) Cells with 10 or more RAD51 foci were scored for each condition. (C) Representative images of treated primary skin fibroblasts. The cytoplasmic fraction of RAD51 was also scored for each condition (D), as well as cells with 10 or more RPA foci (E). (F) Primary skin fibroblasts were also transfected with 2XMBP-scFv expression constructs and then irradiated with 10Gy IR and fixed for immunofluorescence. (G) Cells with 10 or more RAD51 foci were scored for each condition. (H) Representative images of transfected primary skin fibroblasts. The cytoplasmic fraction of RAD51 (I) was also scored, in addition to cells with 10 or more RPA foci (J). Error bars represent the SEM; ****P < 0.0001, ***P < 0.001, **P < 0.01, and *P < 0.05 by unpaired t-test.