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. 2017 Sep 13;45(20):11908–11924. doi: 10.1093/nar/gkx827

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 3. — Bulk topoisomerase assays show that the activity of TopA depends on the presence of the lysine repeats in the C-terminal domain. (A) Images of agarose gel-based assays demonstrating the activity of TopA, TopACTD_Ec, TopA881 and TopAED:AA (200 nM); the reaction mix was sampled at time points from 0 to 12 min. (B) Estimation of kinetic parameters based on the agarose gel assays. V_max and K_m were calculated by fitting the data for TopA (red), TopACTD_Ec (blue), TopA881 (green) and TopAED:AA (purple) to the Michaelis–Menten equation.