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GTI-seq verifies converse regulation of non-AUG translation by eIF5 and 5MP1 genome-wide. The proportion of translation initiation sites (TIS) with indicated start codons (color-code to the right) is shown for HEK293T transfected with eIF5 or 5MP1-expression plasmid, both (BOTH), or with the vector control (Ctrl). (A) uTIS, TIS located upstream of authentic TIS. (B) All predicted TIS (aTIS) including uTIS.
