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. 2017 Aug 30;45(20):11700–11710. doi: 10.1093/nar/gkx775

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — PP32 and SET/TAF-Iβ associates with newly synthesized histone H4. (A) Scheme illustrating the procedure utilized to label and isolate newly synthesized proteins. (B) Western blot analysis of 1% of the input material (left), purified Flag-immunoprecipitated proteins (middle), and Streptavidin–agarose pulled-down proteins derived from the Flag-immunoprecipitated material (right), as indicated. Top panels were developed with Streptavidin-HRP to visualize AHA-labeled proteins conjugated to biotin AHA–biotin. Bottom panels were developed as indicated. (C) Western blot analysis of 1% of the input material, purified Flag-immunoprecipitated proteins and Streptavidin–agarose pulled-down proteins derived from the Flag-immunoprecipitated material, with extracts in which the CLICK-IT procedure was performed with DMSO instead of AHA. Top panel was developed with Streptavidin–HRP to visualize AHA-labeled proteins conjugated to biotin AHA–biotin. Bottom panels were developed as indicated.