Fig. 3.

Glycome profiling of cell wall extracts of root segments from root apex to 5 cm divided into 1-cm segments (numbered 1–5). Cell walls of each segment were subjected to sequential extraction with 490 mm sodium chlorite, 0·5 % ammonium oxalate, 0·1 m NaOH, 1 m NaOH and 4 m NaOH. Solubilized extracts were screened for an array of plant glycan-directed monoclonal antibodies (Pattathil et al., 2010, 2012) using ELISA. Absorbances are represented according to the colour key on the right. Antibodies are grouped according to the polysaccharides predominantly recognized (coloured boxes): non-fucosylated xyloglucan (NON-FUC-1-6), fucosylated xyloglucan (FUC XG), xylan/xyloglucan (XYLAN-1/XG), XYLAN-2-7, GALACTOMANNAN-I-2, ACETYLATED MANNAN, β-GLUCAN, homogalacturonan backbone (HG BACKBONE-1 and -2), rhamnogalacturonan backbone (RGI BACKBONE), linseed mucilage rhamnogalacturonan (LINSEED MUCILAGE RG-I), Physcomitrella pectin, rhamnogalacturonan-I (RG-Ia, b and c), rhamnogalacturonan-I/arabinogalactan (RG-I/AG), arabinogalactan (AG-1 to -4).