Immunofluorescence labelling of cross-sections of root segments S1 (A, D), S3 (B, E) and S5 (C, F) using β-glucan-directed antibody (BG1). Labelling in sections initiates in all cell walls in S1, fades at the cortex in S3, and practically disappears from the cortex in S5, although it persists in peripheral tissues (pe) (epidermis, exodermis and sclerenchymatous cylinder) and vascular cylinder (vc). Endodermis (en) is not labelled in all sections. Controls (A–C) were stained with toluidine blue. Scale bar = 100 µm.