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. 2017 Nov 23;20(1):12–24. doi: 10.1016/j.neo.2017.10.004

Figure 1.

Figure 1

Loss of PTEN results in increased invasion and migration. (A) Western blot confirmation of stable PANC-1 shPTEN cell pool as compared to Scramble control. (B) PANC-1 Scramble and shPTEN cells were plated, and a scratch assay was performed in both serum-free (top) and serum-containing (bottom) conditions. PANC-1 shPTEN cells were able to move across the scratch significantly farther than PANC-1 Scramble cells in both conditions. (C) PANC-1 cells were cultured in PANC-1 EpM or CAFM and harvested at various timepoints. Lysates were analyzed via Western blot, and it was seen that PANC-1 cells cultured in CAFM expressed less PTEN as time went on, which was accompanied by a robust upregulation of P-AKT. (D) PANC-1 Scramble and shPTEN cells were plated, and a scratch assay was performed in either EpM or CAFM conditions. PANC-1 shPTEN cells traveled significantly farther across the scratch, and that phenomenon was augmented in the presence of CAFM, in which the PANC-1 shPTEN cells traveled more than two-fold farther than PANC-1 Scramble control cells. (E) PANC-1 Scramble and shPTEN cells were plated, and a Transwell invasion assay was performed in either EpM or CAFM conditions. PANC-1 shPTEN cells invaded significantly more through the Transwell membrane; however, that significance was increased in shPTEN cells cultured in CAFM. (F) Western blot analysis of PANC-1 Scramble and shPTEN cells shows that MTSS1 expression decreases in PANC-1 shPTEN cells. (G) Western blot analysis of both metastatic (metPDAC.1) and primary (primPDAC.1) PDAC patient cell lysates shows metastatic patient sample containing decreased PTEN and MTSS1 expression. *P value < .05, **P value < .001, ***P value < .0001.