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. 2017 Nov 23;20(1):12–24. doi: 10.1016/j.neo.2017.10.004

Figure 5.

Figure 5

Increase in MTSS1 expression results in decreased invasion and migration in PTEN-deficient cells. (A) PANC-1 cells were stably transduced with either Scramble control vector or shPTEN knockdown plasmid. PANC-1 shPTEN cells were then transiently transfected with Myc-MTSS1 plasmid, and MTSS1 overexpression was confirmed via Western blot. (B) PANC-1 Scramble, shPTEN, and shPTEN + MOE cells were plated, and a scratch assay was performed in both serum-free (top) and serum-containing (bottom) conditions. Cells that were deficient in PTEN expression moved significantly further across the wound in both conditions, but upon overexpression of MTSS1 in those same cells, there was no significant difference denoted when compared to Scramble control. (C) PANC-1 Scramble, shPTEN, and shPTEN + MOE cells were plated, and a Transwell invasion assay was performed in the presence of Matrigel. PANC-1 shPTEN cells invaded through the membrane significantly more often than Scramble control until MTSS1 was overexpressed, and then no significant difference was seen. *P value < .05, **P value < .001.