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Western blot showing implications of mutating PTEN lipid phosphatase activity, leaving only its protein phosphatase activity as viable. As can be seen in lanes 1 and 3, cells with mutated lipid phosphatase activity (PTENG129E) not only were able to maintain Mtss1 levels but in fact were able to moderately increase them when compared to the control P577 Pten−/− cells in lane 1.
