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. 2017 Nov 20;13(11):e1006736. doi: 10.1371/journal.ppat.1006736

Fig 1. Correlation between progesterone or IFN-γ production with IFN-γ gene methylation in human CD8 Tm cells.

Fig 1

CD8 Tm cells were purified from PBMCs of 10 subjects at before, weeks 14 and 28 of pregnancy and around 1 year after delivery. Average percentages of DNA methylation at 6 CpG sites of IFN-γ gene promoter region are shown in (A). (B) Correlation between serum progesterone levels and IFN-γ gene methylation levels of all samples as shown in (A). Frequencies of IFN-γ-producing PBMC CD8 Tm cells after ex vivo stimulation with PMA and Ionomycin are shown in (C). And Correlation between IFN-γ gene methylation levels and frequencies of IFN-γ-producing CD8 Tm cells is shown in (D). Horizontal lines in (A) and (C) represent median values. One-way ANOVA and Tukey’s multiple comparisons test was used to compare between multiple groups. Pearson correlation analysis was used to determine the potential correlation between two parameters. * P<0.05; ** P<0.01; *** P<0.001. The experiments were performed once.