Figure 1.
Temporal Hierarchy of BM Component Expression during Embryogenesis
(A) Time-lapse imaging of Drosophila embryos expressing GFP-tagged BM components Laminin α (LanA), Collagen IV (Col IV), and Perlecan (Perl). Scale bar, 100 μm.
(B) Quantification of embryonic fluorescence of BM components. Note that LanA is expressed first, followed by Col IV, and finally Perl. Mean ± SEM.
(C) Quantification of embryonic fluorescence of BM components in control and srpAS embryos. Mean ± SEM. Insets show quantification of the maximum fluorescence in each single embryo. Bars in the insets indicate median ± interquartile range.
(D) Localization of GFP-tagged BM proteins on the ventral surface of the nerve cord in mutant backgrounds of opposite components. LanA-GFP and Col IV-GFP were imaged at stage 15, while Perl-GFP was imaged at stage 17 of development. Scale bar, 20 μm.
(E) Quantification of embryonic fluorescence of GFP-tagged BM components in mutant backgrounds. Bars indicate median ± interquartile range.