Figure 2.
During BM Deposition, LanA Is Diffusing in the Embryonic Hemocoel while Col IV and Perl Are Locally Deposited
(A) Confocal imaging of GFP-tagged BM proteins (green) and hemocytes (magenta) during development. Scale bar, 50 μm.
(B) High-magnification images of LanA-GFP (stage 15), secreted-GFP (SecrGFP) (stage 15), Col IV-GFP (stage 16), and Perl-GFP (stage 17). Scale bar, 10 μm. Graphs are line scans of BM component fluorescence with arrows highlighting the border of the hemocyte cell body.
(C) FRAP analysis of secreted-GFP, LanA-GFP, and Col IV-GFP in stage 15 embryos at the midline of the VNC. Mean ± SEM. Scale bar, 10 μm.
(D) TEM of the ventral hemocoel of a stage 15 embryo, with the hemocyte highlighted in blue. Note the hemocyte squeezing between the epithelium and VNC within the narrow hemocoel. Scale bar, 10 μm.
(E) TEM of a stage 16 hemocyte migrating along the VNC reveals BM material (arrows) beneath migrating cells. Scale bar, 2 μm.
(F and G) Time-lapse imaging by lattice light-sheet microscopy of Col IV deposition (green) and hemocytes (magenta) at stage 14 of embryogenesis, as hemocytes migrate laterally from the ventral midline. (F) Low magnification. (G) High-magnification view highlighting the correlation between the leading edges of hemocytes and Col IV deposition. Scale bars, 10 μm.