Figure 2.

Deficiency mapping of lys¹. (A) A schematic diagram illustrating the size and position of the molecularly-defined deficiencies used to genetically map lys¹. Homozygous lys¹ females were crossed with deficiencies (Df) that span the genomic region to the left of dachs (d). Deficiencies highlighted in red failed to complement lys¹. (B) GC-MS was used to measure relative lysine levels in 3-day-old adult males. Animals that were heterozygous for either lys¹ or any of the examined deficiencies exhibited similar lysine levels. In contrast, homozygous lys¹ mutants and males that were trans-heterozygous for lys¹ and either Df(2L)ED508, Df(2L)ED12527, or Df(2L)BSC142 displayed lysine levels that were up to 50-fold higher than controls. Each data point represents a single sample that contained 25 adult male flies. GC-MS, gas chromatography-mass spectrometry.