Table 1. Profile of the mutations suppressing the inability of FurE-ΔC to grow on uric acid. The relevant nucleobase substitution is underlined.
| Mutation | Codon Change | Number of Isolates | Location | Putative Domain |
|---|---|---|---|---|
| D26N | GAC > AAC | 1 | N-tail | Flex loop |
| T133V | ACG > GTG | 4 | TMS3 | Binding site filter |
| G222K | GGG > AAG | 1 | L5 | Flex loop |
| GGG > AAG | 4 | |||
| N308T | AAC > ACC | 7 | L7 | Flex loop |
| AAC > ACA | 1 | |||
| AAC > ACT | 1 | |||
| S296R | AGC >CGC | 4 | TMS7 | Gate |
| V343I | GTC > ATC | 1 | TMS8 | Binding site filter |
| I371L | ATC > CTC | 1 | TMS9 | Gate |
| I371F | ATC > TTC | 3 | TMS9 | Gate |
| I371P | ATC > CCC | 1 | TMS9 | Gate |
| Y392N | TAC > AAC | 6 | TMS10 | Gate |
| Y392C | TAC > TGC | 5 | TMS10 | Gate |
| Y392E | TAC > GAA | 1 | TMS10 | Gate |
| L394P | CTG > CCG | 4 | TMS10 | Gate |
TMS, transmembrane segment.