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Ty1 IN and Gag levels in ribosome biogenesis and RNA localization mutants. Total cell extracts from CNC− and CNC+ wild type and loc1Δ, rps0bΔ, rpl7aΔ, she2Δ, and puf6Δ mutant strains expressing pBDG606 were subjected to western blot analysis using IN and VLP antisera. Pgk1 served as a loading control. Refer to Figure 1 for a description of Ty1 proteins.
