Figure 1.

Yeast two-hybrid assays detect interactions between ADA2a and ADA2b and INO. (A, D, and G) Maps showing which proteins were produced in yeast strains streaked on the adjacent plates. “AD” and “BD” indicate the activation and DNA-binding domains of yeast GAL4, respectively. (B, E, and H) Streaks of indicated yeast strains on media lacking leucine and tryptophan, selecting only for the presence of the two expression plasmids in each strain. (C, F, and I) Streaks of indicated yeast strains on media lacking leucine, tryptophan, adenine, and histidine selected for interaction of the indicated proteins. The results show that none of the tested constructs significantly auto-activated, but that both AD-ADA2a and AD-ADA2b productively interact with both BD-INO and BD-INOΔN. A possible weak interaction of the ADA2 fusions with BD-INOΔC is also visible.