Figure 2.
Accumulation of ADA2b-GFP (PRZ-GFP) fusion in ovule cells. The expression pattern of a P-PRZ1::PRZ1:GFP (P-ADA2b::ADA2b:GFP) fusion in a genomic fragment that complemented the prz1 mutant (Sieberer et al. 2003; Anzola et al. 2010) was evaluated by laser confocal microscopy. Left panels are GFP fluorescence and the right panels are the fluorescent images overlaid on differential interference contrast images. Images are of ovules at stages: (A and D) 2-I; (B and E) 2-IV; and (C and F) 4-V. Expression is in all cells at all stages of ovule development and the fusion protein accumulates in the nucleus. Arrowhead, site of outer integument initiation; i, inner integument; n, nucellus; o, outer integument; and op, ovule primordium. Bar, 25 µm.