Figure 7.

Oxidative stress response and antioxidant-capacity phenotypes of Gclc⁴⁶/Y are not rescued by 20-hydroxyecdysone (20E) administration. (A) The survival rate and developmental progression of control w¹¹¹⁸/Y and Gclc⁴⁶/Y animals that were fed sucrose‐agar medium containing paraquat (PQ) and/or 20E. Second-instar larvae at 48 hr after egg laying (AEL) were transferred to the medium, then fed media for 12 hr. *** P < 0.001 chi-square test with Bonferroni correction. n.s., not significant. (B) Antioxidant capacity of body fluids isolated from control w¹¹¹⁸/Y and Gclc⁴⁶/Y second-instar larvae (60 hr AEL) that were fed standard food supplemented with vehicle ethanol (−), 20E, ascorbic acid (asc.), and glutathione (GSH). * P < 0.05 and ** P < 0.01 by Dunnett’s test.