Figure 6.

Larval-arrest phenotype induced by ethacrynic acid (EA) is rescued by feeding 20-hydroxyecdysone (20E) and cholesterol (CLR). (A–D) The survival rate and developmental progression of wild-type (w¹¹¹⁸) animals that were fed standard food supplemented with vehicle ethanol 0%, (A), 0.1% (B), 0.2% (C), and 0.5% (D) EA. (B’–D’) The survival rate at 192 hr after egg laying (AEL) was examined when vehicle ethanol (−), 20E, and CLR were added with 0.1% (B’), 0.2% (C’), and 0.5% (D’) EA to standard food. L1, L2, and L3 indicate first-, second-, and third-instar larvae, respectively. N = 60.