Figure 1.

Experimental design and RNA-sequencing (RNA-seq) pipeline. (A) D. sechellia flies were reared on cornmeal medium. Adult female flies, 0–4 d old, were exposed to either control food or food containing 0.7% octanoic acid (OA). Total RNA was extracted from replicates of 10 whole adult female flies. (B) RNA-seq pipeline performed in Galaxy. Read quality was checked using FASTQC and aligned to the D. sechellia genome using Bowtie2. Differential gene expression statistical analysis was performed with Cuffdiff. All subsequent processing and visualization was performed in R and gene ontology (GO) term enrichment was performed using the Gene Ontology Consortium.