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. 2017 Oct 17;7(12):3913–3924. doi: 10.1534/g3.117.300339

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Copyright © 2017 Snyder et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Gdt1 promotes Ca²⁺ detoxification independent of Vcx1 and Pmc1. (A) A panel of Saccharomyces cerevisiae strains lacking Gdt1, Vcx1, and Pmc1 in all possible combinations was assayed for Ca²⁺ tolerance in the presence (gray bars) or absence (black bars) of FK506 as described in Figure 2A. (B) A strain expressing epitope-tagged Gdt1-TAP was grown to log phase in YPDS medium containing 200 mM CaCl₂ and/or 1 µg/ml FK506, harvested, lysed, and analyzed by SDS-PAGE and western blotting using anti-TAP polyclonal (top) and anti-tubulin monoclonal (bottom) antibodies.