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. 2017 Oct 17;7(12):3913–3924. doi: 10.1534/g3.117.300339

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Copyright © 2017 Snyder et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Erd1 prevents Pi loss to the environment. The indicated mutant strains were grown to log phase in SC medium containing tracer levels of [32]Pi radioisotope, washed extensively in Pi-free SC medium, and then in the same medium (A and B) or in SC medium (C). At the indicated times of incubation at 30°, aliquots were removed and cell-free supernatants were analyzed by liquid scintillation counting (A and C) or by TLC (B). Charts illustrate the averages (±SD) of three biological replicates.