Figure 4.

Evidence for supercoiling changes in the FusA^A608E-TopA^S180L mutant. (A) Gel picture showing mobility of pUC18 topoisomers on agarose gel containing 2.5 µg/ml chloroquine. Positions of negatively supercoiled and relaxed forms of the plasmid are indicated by a schematic. (B) Scatter plot showing correlation of log₂ fold changes of genes in the FusA^A608E-TopA^S180L mutant with microarray derived gene-expression ratios obtained by inhibiting DNA gyrase function using 20 µg/ml novobiocin (data obtained from Peter et al. 2004). For a detailed comparison with the Peter et al. (2004) dataset, refer to Figure S13 in File S2.