Figure 6.

Effect of second site mutations on gene expression and dependence on EF-G. Scatter plots comparing log₂ fold changes of differentially expressed genes among mutants. Mutants are referred to by their gene names for brevity. Gray zones indicate the region between the log₂ fold changes of +1 and −1 (corresponding to fold changes of 2 and 0.5), and thus highlights the region of low/no fold change. Red points show genes upregulated and blue points show genes downregulated in the relevant second site single mutant. (A–C) Fold changes of genes differentially expressed in the CyaA^N600Y mutant were compared with the FusA^A608E-CyaA^N600Y and FusA^A608E mutants. (D–F) Fold changes of genes differentially expressed in the CpxA^F218Y mutant were compared with the FusA^A608E-CpxA^F218Y and FusA^A608E mutants. (G–I) Fold changes of genes differentially expressed in the RpoD^L261Q mutant were compared with the FusA^A608E-RpoD^L261Q and FusA^A608E mutants.