TABLE 2.
Fold Changes and Trends of Serum Metabolomics Data
| Metabolite | m/z_RT | ESI and adduct | Proposed pathway/description | ppm error | 1 Gy neutron/Control | 1 Gy X ray/Control | 1 Gy neutron/1 Gy X ray | Trend between 1 Gy exposed (neutron/X ray) | P value 1 Gy comparisons | FDR value |
|---|---|---|---|---|---|---|---|---|---|---|
| Day 1 | ||||||||||
| L-tyrosine | 182.0816_0.36 | [M + H]+ | Amino acid | 2.7 | −1.8 | −1.2 | −1.5 | ê | 0.04 | 0.23 |
| L-glutamic acid | 146.0460_0.34 | [M − H]− | Arginine biosynthesis and glutathione metabolism | 0.9 | −2.3 | −1.5 | −1.5 | ê | 0.09 | 0.34 |
| Taurine | 124.0068_0.33 | [M − H]− | Bile acid metabolism | 4.3 | −1.2 | 1.0 | −1.1 | – | 0.05 | 0.25 |
| Succinic acid | 117.0187_0.38 | [M − H]− | Dicarboxylic acid | 4.7 | −1.4 | 1.1 | −1.5 | ê | 0.01 | 0.12 |
| L-carnitine | 162.1129_0.33 | [M + H]+ | Fatty acid β-oxidation | 2.7 | 1.3 | 1.0 | 1.3 | é | 0.006 | 0.09 |
| Pyroglutamic acid | 128.0346_0.36 | [M − H]− | Glutathione metabolism | 4.8 | −2.0 | −1.3 | −1.6 | ê | 0.03 | 0.18 |
| Eisosapentaenoic acid | 303.2327_8.09 | [M + H]+ | Omega-3 fatty acid | 2.9 | −3.4 | 1.1 | −3.7 | ê | <0.001 | 0.02 |
| Linoleic acid | 279.2323_8.41 | [M − H]− | Omega-6 fatty acid | 2 | −4.8 | −1.5 | −3.2 | ê | 0.003 | 0.07 |
| Arachidonic acid | 303.2322_8.34 | [M − H]− | Polyunsaturated fatty acid | 2.4 | −1.5 | −1.2 | −1.3 | ê | 0.14 | 0.41 |
| Arachidonic acid | 305.2489_8.34 | [M + H]+ | Polyunsaturated fatty acid | 4.8 | −1.4 | −1.1 | −1.3 | ê | 0.02 | 0.17 |
| Uric acid | 167.0204_0.34 | [M − H]− | Pyrimidine metabolism | 3.5 | −1.4 | −1.1 | −1.4 | ê | 0.002 | 0.05 |
| Sphinganine-1-phosphate | 380.2566_7.10 | [M − H]− | Sphingolipid metabolism | 1.2 | 1.0 | −1.4 | 1.4 | é | 0.04 | 0.23 |
| Citric acid | 191.0192_0.38 | [M − H]− | TCA cycle | 2.7 | −1.5 | −1.3 | −1.2 | ê | 0.22 | 0.51 |
| Day 7 | ||||||||||
| L-tyrosine | 182.0816_0.36 | [M + H]+ | Amino acid | 2.7 | −1.1 | 1.0 | −1.2 | ê | 0.54 | 0.91 |
| L-glutamic acid | 146.0460_0.34 | [M − H]− | Arginine biosynthesis and glutathione metabolism | 0.9 | −1.6 | −1.1 | −1.4 | ê | 0.25 | 0.80 |
| Taurine | 124.0068_0.33 | [M − H]− | Bile acid metabolism | 4.3 | −1.6 | −1.2 | −1.3 | ê | 0.05 | 0.64 |
| Succinic acid | 117.0187_0.38 | [M − H]− | Dicarboxylic acid | 4.7 | −1.1 | −1.2 | 1.0 | – | 0.87 | 0.99 |
| L-carnitine | 162.1129_0.33 | [M + H]+ | Fatty acid β-oxidation | 2.7 | 1.1 | −1.1 | 1.1 | – | 0.05 | 0.65 |
| Pyroglutamic acid | 128.0346_0.36 | [M − H]− | Glutathione metabolism | 4.8 | −1.2 | −1.4 | 1.1 | – | 0.49 | 0.90 |
| Eisosapentaenoic acid | 303.2327_8.09 | [M + H]+ | Omega-3 fatty acid | 2.9 | 1.3 | −1.6 | 2.1 | é | 0.03 | 0.64 |
| Linoleic acid | 279.2323_8.41 | [M − H]− | Omega-6 fatty acid | 2 | 1.0 | −1.4 | 1.4 | é | 0.52 | 0.90 |
| Arachidonic acid | 303.2322_8.34 | [M − H]− | Polyunsaturated fatty acid | 2.4 | 1.3 | −1.1 | 1.4 | é | 0.18 | 0.76 |
| Arachidonic acid | 305.2489_8.34 | [M + H]+ | Polyunsaturated fatty acid | 4.8 | 1.2 | −1.1 | 1.3 | é | 0.09 | 0.69 |
| Uric acid | 167.0204_0.34 | [M − H]− | Pyrimidine metabolism | 3.5 | −1.3 | −1.2 | −1.1 | – | 0.45 | 0.90 |
| Sphinganine-1-phosphate | 380.2566_7.10 | [M − H]− | Sphingolipid metabolism | 1.2 | −1.5 | 1.1 | −1.6 | ê | 0.05 | 0.64 |
| Citric acid | 191.0192_0.38 | [M − H]− | TCA cycle | 2.7 | −1.1 | 1.0 | −1.1 | – | 0.43 | 0.76 |