Figure 4.

Acute Hlf Depletion and Modulation of Hlf into a Transcriptional Repressor Lead to Enhanced Lymphopoiesis

Hlf-inducible GMLPs (CD45.1⁺) were transplanted into sublethally irradiated WT recipients (CD45.2⁺) on DOX food. After 14 days, one group of mice was shifted to normal food and kept for another 8 days prior to analysis (“Hlf depleted”), while control mice were treated with DOX throughout the experiment (“Hlf induced,” n = 7 and 6 mice, respectively, from one experiment).

(A) Data show the percentage of the indicated donor-derived cell types out of all CD45⁺ cells.

(B and C) The impact of Hlf target gene silencing on differentiation was studied by culturing control or M33-Hlf retrovirus infected WT GMLPs in B/NK and T cell permissive conditions (n = 6 replicates per group from two experiments). Shown is the lineage output relative to controls of indicated subsets in OP9 cultures (B) and OP9-DL1 cultures (C), respectively.

Error bars denote SEM.