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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Neuropharmacology. 2017 Nov 14;129:84–99. doi: 10.1016/j.neuropharm.2017.11.019

Figure 9.

Figure 9

Effect of minocycline on ethanol-induced neuronal death in co-cultures of mouse cortical neurons and microglia. Co-cultures of SIM-A9 cells and primary cortical neurons were established as described in the Materials and Methods. Co-cultures were exposed to ethanol (0 or 0.4%) exposure with or without minocycline (50 μM) for 48 hours. The viability of neurons was determined by MTT as described in the Materials and Methods. Data were the mean ± SEM of three independent experiments. &p < 0.05, statistically significant difference from cortical neuron alone; $p < 0.05, statistically significant difference from cortical neuron with ethanol exposure; *p < 0.05, statistically significant difference from co-culture group without ethanol; #p < 0.05, statistically significant difference from ethanol group in co-culture. CN: Cortical neuron; CC: Co-culture.