Figure 7.

PSMB5 knockdown by shRNA significantly attenuated MDA-MB231 breast cancer proliferation and migration in vitro and in vivo. (A) The proliferation rates of the MDA-MB231 luciferase control cell lines and stable shPSMB5 knockdown MDA-MB231 cell line were determined by colony formation assay (B) the static data of colony formation assay. Values are the average of assays performed in triplicate. Error bars represent SD (n=3). *p < 0.05. (C) Motility of shPSMB5 knockdown as well as luciferase control MDA-MB231 cells were evaluated by Boyden chamber migration assays. (D) Quantification of random motility to PSMB5 knockdown and luciferase control MDA-MB231 cells with boyden chamber assay Error bars represent SD (n=3). *p < 0.05. (E) 1 × 10⁶ MDA-MB231 cells were subcutaneous injected into NOD-SCID mice and the mice were treated with shPSMB5 or luciferase shRNA lentivirus once a week, tumor volume was calculated using the following formula: tumor volume (millimeters cubed) = L × W²/2, where L is the length, and W is the width (n=5 for each group, P=0.016).