Figure 1.

miR-21 promotes glucose uptake in NSCLC Cells. A549 cells were transfected with miR-negative control (miR-NC) or miR-21 for 24 hr. The relative expression of miR-21 was determined by qPCR. 95D cells were transfected with anti-negative control (anti-NC) or anti-miR-21 for 24 hr. The relative expression of miR-21 was detected by qPCR. Glucose uptake was measured in control and miR-21-overexpressing A549 cells. Glucose uptake was measured in control and miR-21-knockdown 95D cells. Cells were deprived for glucose for 12 hr followed by glucose stimulation for additional 3 hr. TXNIP expression was examined in control and miR-21-overexpressing A549 cells by western blotting. Cells were deprived for glucose for 12 hr followed by glucose stimulation for additional 3 hr. TXNIP expression was examined in control and miR-21-knockdown 95D cells by western blotting. Data are shown as mean ± SD in three separate experiments in triplicate. *p < 0.05.