Figure 6.

Restoring FBP1 expression reverses the effects induced by miR-21 overexpression in A549 cells. A549 cells were transfected with miR-21. Subsequently, FBP1 was transfected into miR-21-A549 cells. The expression of FBP1 was shown by Western blot assay. Glucose uptake was measured in indicated cells. Lactate excretion was measured in indicated cells. Cell growth under hypoxic condition was measured by cell-count assay for 2 days. Data are presented as a percentage of miR-NC control values for 95D cells. Data are shown as mean ± SD in three separate experiments in triplicate. *p < 0.05.