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. 2017 Nov 1;7(11):2131–2143.

Figure 4.

Figure 4

CKS1 knockdown suppressed proliferation and cell cycle progression of glioma cells. A. Western blot showing the levels of CKS1, p-AKT, AKT, p-ERK1/2 and ERK1/2 in U87 and U251 cells after transfection with siCKS1. B, C. Proliferation ability was determined using the CCK-8 assay following culture for 96 h. Data are presented as the means of triplicate experiments. D, E. Long-term cell viability was evaluated using the colony formation assay. Data are presented as the means of triplicate experiments. F, G. Proliferating cells were examined using the EDU assay. Representative images are shown (original magnification, 200 ×). (**P<0.01).