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. 2017 Nov 1;7(11):2131–2143.

Figure 5.

Figure 5

CKS1 reintroduction reverses the inhibitory effect of miR-936. A, B. Western blot analysis of CKS1, p-AKT, AKT, p-ERK1/2 and ERK1/2 in the indicated cells. C, D. CCK8 assay with miR-control or miR-936-transduced U87 and U251 cells transfected with vector or CKS1; **P<0.01 and ***P<0.001. E, F. Co-transfected U87 and U251 cells were analyzed by the colony formation assay; *P<0.05 and **P<0.01. G-I. The Edu assay was performed 48 h after cotransfection; *P<0.05 and **P<0.01. J-L. Flow cytometry was conducted to determine the cell cycle distribution in each group.